F1 Hybrid Bl6/Bl-Swiss Feederless ES Cell Line
Robust F1 hybrid embryonic stem cell line from Mus musculus (lab mouse), generated by crossing C56BL/6J mice to Black-Swiss mice. This ES cell line does not require feeder cells for propagation and readily transmits through the germline using outbred CD1 4-8 cell embryos as hosts.
Applications
- Streamlined generation of genetically modified founder mice with >50% c57BL/6J genetic background.
Unique features
- Efficiently generates high percentage chimeras
- Compatible with inexpensive outbred CD1 embryos
- Eliminates need to use inbred albino C57 embryos as is common practice
- Albino CD1 host embryos allow assessment of chimeras by black on white coat color
- Germline transmission of all mutations targeted and tested to date
- Feeder cell independent propagation enables straightforward maintenance and manipulation
Research area
ES cell genome engineering
Alternate reagents
Available C57BL/6J ES cell lines are extremely difficult to work with, and they are not as successful at contributing to the germline of chimeras
Publications/references
None
Other sources of validation
International Society for Stem Cell Research Annual meeting (ISSCR 2020) – Boston/virtual meeting June 2020
Principal investigator
Learn more
To learn more about this cell line, please email Kamya Rajaram.