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F1 Hybrid Bl6/Bl-Swiss Feederless ES Cell Line

Robust F1 hybrid embryonic stem cell line from Mus musculus (lab mouse), generated by crossing C56BL/6J mice to Black-Swiss mice. This ES cell line does not require feeder cells for propagation and readily transmits through the germline using outbred CD1 4-8 cell embryos as hosts.

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Applications

  • Streamlined generation of genetically modified founder mice with >50% c57BL/6J genetic background.

Unique features

  • Efficiently generates high percentage chimeras
  • Compatible with inexpensive outbred CD1 embryos
  • Eliminates need to use inbred albino C57 embryos as is common practice
  • Albino CD1 host embryos allow assessment of chimeras by black on white coat color
  • Germline transmission of all mutations targeted and tested to date
  • Feeder cell independent propagation enables straightforward maintenance and manipulation

Research area

ES cell genome engineering

Alternate reagents

Available C57BL/6J ES cell lines are extremely difficult to work with, and they are not as successful at contributing to the germline of chimeras

Publications/references

None

Other sources of validation

International Society for Stem Cell Research Annual meeting (ISSCR 2020) – Boston/virtual meeting June 2020

Principal investigator

Kathleen J Millen, PhD

Learn more

To learn more about this cell line, please email Kamya Rajaram.

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